Molecular uniqueness of resting NK cells

(a) Heat map of genes with the most significant difference in expression in NK cells relative to all other cell populations, presented (as in Fig. 1a) in decreasing order of significance (full list of genes, Supplementary Table 1). (b) Ly49H expression in splenic leukocytes (left; number above bracketed line indicates percent Ly49H+ cells), and expression of NKp46 and TCRγδ by Ly49H+ cells (right; outlined area indicates TCRγδ−NKp46+ cells). (c) Proportion of the 93 NK cell signature genes in a regulated by the transcriptional regulators (encoded by the genes along the horizontal axis) most 'strongly' predicted to do so (significantly linked), presented in decreasing degree of influence (left to right). Data presented are based on the analysis of a minimum of three independent replicates per cell type (a,c) or are representative of three independent experiments (b).